New insights into disease onset

By
dementianews
-
Aβ43 generation in familial Alzheimer's disease: New insights into disease onset
In this study, γ-secretase was extracted from wild type (WT) cells and cells carrying FAD-related genetic mutations (mutations in presenilin 1: L85P, I143T, H163R, L166P, G206V, I213T, M233T, C263F, E273A, E280A, L286V, G384A, and delta E9). The enzyme was mixed with its substrate in vitro to generate Aβ, the amyloid intracellular domain (AICD), and other peptides. Given the varying levels of Aβ in the samples, the data were normalized to AICD levels. The ratio of VIVIT (peptide released during the enzymatic cleavage of Aβ48 to Aβ43) to AICD was correlated with the age of onset. The open circle indicates WT enzyme, and the closed circles indicate FAD mutant enzymes. All experiments were repeated in triplicate. Credit: Nobuto Kakuda from Doshisha University

Alzheimer’s disease (AD)…

Read more…

RELATED ARTICLESMORE FROM AUTHOR