Animals
This study was approved by the La Trobe University Animal Ethics Committee (AEC19026 and AEC16-93). All procedures were performed in accordance with the ARRIVE guidelines and the National Health and Medical Research Council of Australia code for the care and use of animals for scientific purposes. Male C57BL/6J (WT) mice (n = 114; 8–12 weeks old) were obtained from an in house breeding colony. Standard rodent chow and drinking water were provided ad libitum. Animals were housed with littermates in individually ventilated cages. Mice were randomly assigned to treatment groups and investigators were blinded to treatment groups where possible. As young female mice are known to have a blunted response to angiotensin II infusion10, it is not a reliable model to study a potential therapy for hypertension in the female sex. Thus, we only used male mice in this study.
