Animals
All animal experiments were performed in accordance with the United Kingdom Animals (Scientific Procedures) Act of 1986 Home Office regulations and approved by the University of Strathclyde Animal Welfare and Ethical Review Body and the Home Office (PPL70/8883). 5xFAD mice (JAX006554)41 were crossed with PV-IRES-Cre mice (JAX008069), followed by crossing with Ai32 mice (JAX012569). All genotyping was performed by Transnetyx using real-time PCR. Six 4–6 month old animals in both sexes were used in this study: three 5xFAD::PV-Cre::Ai32 mice (1 male, 2 females) and three 5xFAD::PV-Cre (2 males and 1 female). Two PV-Cre::Ai32 mice (female, 4 months old) were used for simultaneous recording of the BF and cortical population activity while optogeneticially stimulating BF PV+ neurons (Supplementary Fig. S1). All animals were housed individually in high-roofed cages with a…
