HDAC1 modulates OGG1-initiated oxidative DNA damage repair in the aging brain and Alzheimer’s disease

Mouse strains

All mouse work was approved by the Committee for Animal Care of the Division of Comparative Medicine at Massachusetts Institute of Technology. Hdac1^f/f mice were gifted from E.N. Olson²³. Hdac1^f/f mice were bred with Nestin-Cre transgenic mice²⁴ to generate Hdac1 brain-specific conditional knockout (Hdac1 cKO) mice. 5XFAD mice were obtained from Jackson Laboratory (JAX ID# 34840), and were crossed with Hdac1 cKO mice to generate Hdac1 cKO; 5XFAD (compound transgenic) mice. Aged C57BL/6J mice were obtained from National Institute on Aging. Two- to three-month-old male mice were used as young group, and 13- to 17-month-old male mice were used as aged group. Mice were housed in groups of three to five animals on a standard 12 h light/12 h dark cycle, temperature (24 °C), and humidity (45%) with food and water available ad libitum. All experiments were performed…