Characterization of lactic acid bacteria derived exopolysaccharides for use as a defined neuroprotective agent against amyloid beta 1–42 -induced apoptosis in SH-SY5Y cells

The identification of the isolates was done via sequencing each one’s 16 S rRNA gene region, resulting in the amplification of a fragment of 1492 bp. The isolates were shown to be lactobacilli and were ≥ 99% similar to strain types deposited in the GenBank database (gene bank accession number FJ915697.1 and AB112083.1). As the results of the sequence analysis, isolates were identified as Lactobacillus delbrueckii ssp. bulgaricus B3, and Lactobacillus plantarum GD2.

The amounts of l-EPSs of L. delbrueckii ssp. bulgaricus B3 and L. plantarum GD2 produced were 435 and 385 mg/L, respectively. L. delbrueckii ssp. bulgaricus B3 produced the highest amount of l-EPS. In previous studies, the amount of EPS varies from 25 to 132 mg/L for L. lactis ssp. cremoris and L. rhamnosus C83, and 130 to 250 mg/L for L. casei CG11 and L. delbrueckii ssp. bulgaricus NCFB 2772, respectively^29,