Therapeutic B-cell depletion reverses progression of Alzheimer’s disease

Mice

The animal protocols were approved and permission was granted to perform animal experiments by the ACUC committee of the National Institute on Aging (ASP 321-LMBI-2022) under the Guide for the Care and Use of Laboratory Animals (NIH Publication No. 86-23, 1985). AD transgenic mice (5–80 weeks of age, females) were bred, aged, and housed in the same, specific pathogen-free environment at the National Institute on Aging (NIA). Female C57BL/6j mice (Stock # 000664) were purchased from Jackson Laboratory (Bar Harbor, ME) and congenic 3×TgAD mice (triple transgenic with three human genes associated with familial AD, B6;129-Psen1^tm1Mpm Tg(APPSwe,tauP301L)1 Lfa/Mmjax))^24,25, APP/PS1 mice (B6.Cg-Tg(APPswe,PSEN1DE9) 85Dbo/J)¹⁶, 5×FAD mice expressing mutant human APP and PSEN1 genes (B6.Cg-Tg;APPSwFILon,PSEN1*M146L*L286V)²¹ and J_HT mice (B6.129P2-Igh-J^tm1Cgn/J), which do not develop…