Amyloid precursor protein-b facilitates cell adhesion during early development in zebrafish

Animal care and ethics statement

Fish were maintained in stand-alone racks on a 14h:10h light:dark cycle at 28.5 °C, at the facility of the Institute of Neuroscience and Physiology, University of Gothenburg. System water was kept at a pH of 7.2–7.5 and conductivity of 600μS. Larvae were raised in embryo medium (EM) (1.0 mM MgSO₄, 0.15 mM KH₂PO_4, 0.042 mM Na₂HPO_4, 1 mM CaCl_2, 0.5 mM KCl, 15 mM NaCl, 0.7 mM NaHCO₃) at 28.5 °C in dark except for those in behavior tests that were maintained at a 14 h:10 h light:dark cycle. This study was approved by the Animal ethical committee in Gothenburg. All procedures for the experiments were performed in accordance with the animal welfare guidelines of the Swedish National Board for Laboratory Animals.

Mutagenesis using the CRISPR/Cas9 system

The CRISPR/Cas9 system was used to generate zebrafish mutants, as previously…