Materials
Thiobarbituric acid (TBA), metaphosphoric acid, 2,7-dichlorodihydrofluorescein diacetate (DCF-DA), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetra-zolium bromide (MTT), 5,5,6,6-tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide (JC-1), lactate dehydrogenase (LDH) assay kit, superoxide dismutase (SOD) determination kit, adenosine triphosphate (ATP) bioluminescence assay kit and all other chemicals used were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). A total glutathione (GSH) kit was purchased from Enzo Life Science Inc. (Farmingdale, NY, USA).
Preparation of samples
The A. arguta fruit (cultivar: Autumn Sense) was obtained from the National Institute of Forest Science (Suwon, Korea) in September 2013. A. arguta was extracted in 40% ethanol at 40 °C for 2 h. The extract obtained was filtered, concentrated using a rotary evaporator, and…
