Generation of the transgenic mouse line carrying the Aβ1-42-GFP sequence together with the β-actin promoter
Transgenic mice expressing human Aβ1-42 fused with GFP were generated using a modified pEGFP-N1 vector (Takara BIO Inc, Shiga, Japan) containing a chicken β-actin promoter (pAct-Aβ1-42-GFP) as described previously31,32. Fertilized eggs from B6C3F1(C57BL/6 N × C3H/HeN) mice received microinjections of the pAct-Aβ1-42-GFP. Three independent stable transgenic lines were obtained and one of them was used in this study. All mice used were males and heterozygous for the transgene. Transgene expression was screened by tail PCR. The B6C3F1 strain exhibited the retinal degeneration phenotype. C57BL/6N has the rd8 mutation which is a single nucleotide deletion in crb1 gene and exhibits a progressive light-colored spot in the fundus of the eye already at 5 weeks of age49,50….
