Sample preparation for DXB
Aβ was dissolved in 0.1% NH4OH/1 mM DMSO to a concentration of 0.1 mg/mL. ATP, ADP, and AMP (10 mM) were added to the Aβ solution and reacted at 37 °C for 0 min, 30 min, 1 day, and 4 days. Aβ solution without reagent as a control was also measured after the same reaction time at 37 °C as when reagent was added.
The immobilization of Aβ molecules onto the substrate film and the labeling of Aβ molecules with gold nanoparticles were performed based on previous studies14. To immobilize Aβ to the polyimide film by amine coupling, 100 μL of NHS/WCS (Dojindo) with minimum essential medium (MEM) buffer was added to the film after KOH exposure. The films were incubated at room temperature (RT) for 10 min. The Aβ solution was dissolved in activation buffer to a concentration of 70 μg/mL. Then, 100 μL of Aβ solution was added to the film and…
