Ethical oversight of animal and human experiments
All animal experimentation was conducted following approval by local authorities of the Federal State of Berlin (X9007/17). All test animals lived in standardized conditions where temperature, humidity, and hours of light and darkness were maintained at a constant level, all year round. The air was approximately 22 °C and the humidity approximately 55%. Light switched on and off automatically, imitating a day-night rhythm.
The human iPSC line BIHi043A-XM001 was used56. All ethical approvals are deposited in the Human Pluripotent Stem Cell Registry (https://hpscreg.eu/) under the accession code #BIHi043-A.
The use of donor blood for isolation of human monocytes was ethically approved by the Aarhus University Blood Bank (Protocol no. 77).
For the analysis and collection of samples from MCI patients, the “Central Denmark Region…
