Animals
Tg-APPswe/PS1dE9 (Tg-APP/PS1) mice, which overexpress mutant forms of human amyloid precursor protein (APP) and presenilin-1 (PS1) genes, were maintained as C57BL6 × C3H F1 hybrids as previously described22,32. After weaning, male and female mice were separately housed with 2-3 animals in a standard clear plastic cage in a temperature (23–24 °C)- and humidity (50–60%)-controlled environment under a 12 h light/dark cycle (lights on from 07:00 to 19:00 h), with ad libitum access to water and food.
Genotyping of Tg-APP/PS1 mice was determined by genomic PCR using the following primers; 5′-CTAGGCCACAGAATTGAAAGATCT-3′ and 5′-GTAGGTGGAAATTCTAGCATCATCC-3′ for WT (324 bp), 5′-AATAGAGAACGGCAGGAGCA-3′ and 5′-GCCATGAGGGCACTAATCAT-3′ for the PS1 gene (324/608 bp), and 5′-AGGACTGACCACTCGACCAG-3′ and 5′-CGGGGGTCTAGTTCTGCAT-3′ for the APP gene…
