Study design
This study was conducted exclusively in mice in compliance with protocols approved by Columbia University’s Institutional Animal Care and Use Committee. All methods reported herein are compliant with ARRIVE guidelines. Each subject underwent a single session of FUS-BBBO, bilaterally targeting the hippocampus. Each subject was administered an IP injection of Gd contrast agent, then underwent dynamic contrast-enhanced (DCE) magnetic resonance imaging (MRI) to measure BBBP, followed by a static T1-weighted MRI to visualize the BBBO one hour after treatment with FUS + μB. Every 24 h following FUS-BBBO the subjects received a new Gd injection, and a static T1-weighted MRI was acquired to track the BBBC timeline. Once the BBB was closed, as determined by the absence of signal enhancement on T1-weighted MRI following Gd injection, the animals were sacrificed, and the…
