Tissue samples
Seven human brain hemispheres (six left, one right) were acquired from the Massachusetts General Hospital Autopsy Suite (45–84 years; 68.33 ± 14.39 (mean ± sd); four males, two females, one unknown; postmortem intervals < 24 h). Autopsy consent was obtained from the legally empowered individual and autopsy tissue was collected only when allowance was made for research purposes. Excess tissue (defined as tissue not required for diagnostic purposes) was made available to investigators under a protocol approved by the IRB of MassGeneralBrigham. Samples were fixed by immersion in 10% formalin. Based on clinical reports, all cases were cognitive controls (cognitively healthy). All cases were screened for comorbidities based on the guidelines for the neuropathologic assessment of Alzheimer’s disease40 by the Massachusetts General Hospital Autopsy Suite. No…
