All the experiments were performed in accordance with the ARRIVE guidelines.
Animals
The animal experiments were approved by the Animal Care and Use Committee of Nagoya City University Graduate School of Medical Sciences (permit number 18149, 19-017H02) and conformed to the guidelines for the use of laboratory animals which was published by the Japanese government (Law No. 105, October 1973). The generation of homozygous HCNP-pp KO mice and littermate HCNP-pp floxed control mice was performed as previously reported36. The animals were housed in specific pathogen-free conditions with a 12 h light/dark cycle (with the lights on from 08:00 to 20:00) and were given free access to food and water.
We used 176 slices from 80 male mice for the electrophysiological experiments for LTP (young generation, 11–17 weeks old; control, n = 17, N = 10, HCNP-pp KO, n = 26, N = 10; adult…
