Animal studies
The Swedish (670/671KM→NL) and Indiana (717 V→F) human APP expressing J20 APPSw/Ind transgenic mouse (B6.Cg-Zbtb20Tg(PDGF-APPSwInd/20lms)/2Mmjax, stock No 006293, Jackson Laboratories, ME, USA) [59] was used for these experiments. J20 sperm and female C57BL/6 J wild-type (WT) mice were used with IVF to generate cohorts of J20 mice and their WT littermates. Mice were generated and aged to 11.5 months at the Institut de Recherche en Immunologie et en Cancérologie (IRIC) at the Université de Montréal. During this aging period, mice were housed 2–4 mice per cage in standard macrolon cages under a 12-h regular light/dark cycle, controlled environmental conditions, and ad libitum access to food and water. Approximately 2–3 weeks prior to experimentation (starting at 12-months of age), mice were transferred to the Lady Davis Institute (Jewish General Hospital,…
