Animals
For the in vivo studies, all experimental procedures were approved by the local ethical committee of Maastricht University for animal experiments in accordance with governmental guidelines (approval codes: 2012-063 and 2018-006; Licensed animal ethical committee Ministry of VWS, GZBIVVB981845). For the long-term memory studies, two cohorts of twenty-four 3-month-old male Wistar rats (Charles River, Sulzfeld, Germany) were used (average body weight at the beginning of the study: cohort 1 BAY-747: 292 g; cohort 2 runcaciguat: 334 g). For the L-NAME studies, forty-eight 4-month-old male Wistar rats were used separated into two batches of twenty-four rats (average body weight at the beginning of the study: batch 1: 541 g; batch 2: 440 g). For the in vivo plasticity study, twenty-four 3-month-old male C57BL/6 mice (Charles River, Sulzfeld, Germany) were used (average body weight…
