Cell culture, HIV-1ADA infection, and treatments
Peripheral blood mononuclear cells (PBMCs) were isolated from healthy women blood donors as part of the NIH/NIGMS SC1 project: “Targeting monocyte/macrophage cathepsin B interactome in HIV-1 neurocognitive disorders”, with approval from the University of Puerto Rico-Medical Sciences Campus Institutional Review Board, Human Research Subjects Protection Office (Protocol #0720116). Written informed consents were obtained from all participants. All the experiments were conducted following institutional guidelines and regulations. The rationale for selecting women donors is that HIV-infected MDM from women secrete higher levels of CATB compared to men (Supplementary Fig. 1). PBMCs were cultured, and MDM were isolated by adherence after 7 days of culture in RPMI supplemented with 20% fetal bovine serum (FBS), 10% human serum, and…
