Skin cells from frontotemporal dementia patients may reveal disease mechanisms and biomarkers for drug research

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dementianews
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Skin cells from frontotemporal dementia patients may reveal disease mechanisms and biomarkers for drug research
Fibroblasts of C9-HRE carriers show unaltered C9orf72 levels, express RNA foci, but do not display DPR proteins. a TaqMan assay from fibroblast RNA for C9orf72 all variants. b TaqMan assay from fibroblast RNA for isoform A specific transcript levels. For (a) and (b), data are shown as mean of data points in one biological replicate ± SEM. One-way ANOVA followed by Sidak’s multiple comparison test was performed. Only p values that were significant in the post hoc test are indicated in the graph. c A representative Western blot of the total protein lysates of fibroblasts from a control subject (Con), FTLD patient without the C9-HRE (C9−), and FTLD patient with the C9-HRE (C9+). Cells were treated with 10 μM lactacystin (Lact.) overnight to block protein degradation through UPS. Poly-ubiquitinated proteins…

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