Quinacrine directly dissociates amyloid plaques in the brain of 5XFAD transgenic mouse model of Alzheimer’s disease

Reagents

Aβ(1–42) peptides were synthesized using solid-phase peptide synthesis as previously reported¹³. Thioflavin T (ThT, catalog# T3516), dimethyl sulfoxide (DMSO, catalog# D8418), BSA (bovine serum albumin, catalog# A3059-100G), RIPA buffer (radio immunoprecipitation assay buffer, catalog# R0278), protease inhibitor (catalog# 11836170001), phosphatase inhibitor (catalog# 04906845001), and 30-kDa molecular weight cut-off filter (catalog# UFC903024) were purchased from Sigma-Aldrich. Primary antibodies used for immunostaining and immunoblotting are as follows: 6E10 (anti-Aβ, catalog# SIG-39320, Covance), GFAP (glial fibrillary acidic protein, catalog# AB5541, Millipore Corporation), Iba-1 (ionized calcium-binding adaptor molecule 1, catalog# MABN92, Millipore Corporation), synaptophysin (catalog# MAB5258, Millipore Corporation), PSD95 (postsynaptic density protein 95, catalog#…