Mice
All animal experiments were approved by the University of Illinois at Chicago Institutional Animal Care and Use Committee, ACC Protocol # 17–123 (Lazarov) and # 16–204 (Minshall). All experiments were performed in accordance with institutional guidelines and regulations.
All mice used in this study were obtained from Jackson Laboratories. Twelve week old db/db and wild-type control & 4–5-month-old male MKR diabetic and FVB control mice were used in this study. FVB/NJ (stock # 001800), MKR (FVB-Tg(Ckm-IGF*K1003R)1Dlr/J, stock #016618), C57B6 (stock #000664), db/db (BKS.Cg-Dock7m + / + Leprdb/J, stock #000642).
Immunohistochemistry
Following perfusion with cold PBS, brains were post-fixed in 4% PFA for 24 h. Brains were rinsed three times with PBS and transferred to 30% sucrose for 24 h. Tissue sections were obtained on a freezing microtome (Leica) where they were…
