Cell cultures and animals
HEK293T, Hela, and BV2 cells were originally from ATCC (Manassas, VA, USA) and maintained in our laboratory. These cells and mouse primary microglial cells were prepared and cultured as previously described19,42,57,58. Trem2 KO mice (in C57BL/6N background) and the corresponding WT control mice were from the UC Davis Knockout Mouse Project (KOMP) repository as described previously59 and were bred in the Animal Centre of Xiamen University. Animal procedures were in accordance with the National Institutes of Health’s Guidelines for the Care and Use of Laboratory Animals and were approved by the Animal Ethics Committee of Xiamen University.
Plasmids
TMEM59-Myc and TMEM59-GFP were constructed previously42. TREM2-Myc, TREM2-GFP, and TREM2-CTF-Myc were also constructed previously60. TMEM59-NTF, TMEM59-CTF, and TREM2-NTF were constructed using the pcDNA3.1-Myc/His…
