Human tissue and data collection
Thirty-two human participants with AD were selected from the Massachusetts Alzheimer’s Disease Research Center Longitudinal Cohort study on the basis of the following criteria: (1) clinically diagnosed with dementia due to probable AD; (2) cognitive status assessed at least three times by a neurologist at the MGH Memory Clinic Unit and scored using CDR-SOB; (3) diagnosis of AD confirmed postmortem by an MGH neuropathologist; (4) Braak Neurofibrillary tangles (NFTs) stage V or VI as determined by the location of NFTs with a total tau immunostain and Bielchowsky’s silver stain16; and (5) the least possible concurrent pathologies. Age of onset, age at death, postmortem interval, sex and APOE genotype were also collected and are listed in Supplementary Table 141. Additional information on the sample size and data exclusion can be found in the Reporting…
