Synergistic depletion of gut microbial consortia, but not individual antibiotics, reduces amyloidosis in APPPS1-21 Alzheimer’s transgenic mice

Animal housing and handling

APPPS1-21 mice in a C57BL6Cj background were obtained from M. Jucker (University of Tubingen, Tubingen, Germany) and were housed in the animal research center facility at the University of Chicago. Mice were housed under standard conditions with ad libitum food and water unless otherwise noted. The APPPS1-21 mice co-express the APP_SWE and PS1_L166P transgenes driven by the neuron-specific Thy1 promoter and exhibit Aβ deposition in the cerebral cortex as early as 6 weeks of age³⁹. We have demonstrated that transgene expression is restricted to the brain and that Aβ peptides are present exclusively in this compartment¹².

All experimental procedures were approved by Institutional Animal Care and Use Committee at the University of Chicago. The experimental methods were carried out in accordance with these guidelines and regulations.

ABX treatment

Antibiotics were…