Animals
Mouse experiments were performed with the approval of the Massachusetts General Hospital Animal Care and under the guidelines of Institutional Animal Care and Use Committees (IACUC, protocol #2004N000047) for the use of experimental animals. Mice used included: (1) APPswe/PSEN1∆E9 double Tg mice (The Jackson laboratory, B6.Cg-Tg(APPswe,PSEN1dE9)85Dbo/Mmjax, MMRRC Cat# 034832-JAX, RRID:MMRRC_034832-JAX) (APP/PS1, 2- to 3-mo-old and 8- to 10-mo-old) of either sex, expressing both human APP gene carrying the Swedish mutation and exon 9 deletion mutation in the PS1 gene along with age-matched Wt-littermate controls; and (2) C57BL/6J males (4- to 5-mo-old, Charles River) for the application of the conditioned media and Ru360. For preparation of the conditioned media, Tg2576 males (Taconic Farms, B6;SJL-Tg(APPSWE)2576Kha, IMSR Cat# TAC:1349, RRID:IMSR_TAC:1349), which heterozygously…
