Liposomal drug delivery of Aphanamixis polystachya leaf extracts and its neurobehavioral activity in mice model

Materials

The leaves of the plant Aphanamixis Polystachya were collected from Dinajpur, Bangladesh. All solvents used in this study were purchased from Sigma Aldrich, India and was of analytical grade. Phospholipid was extracted from chicken egg yolk in-house. Cholesterol used was supplied by Sigma Aldrich, India. Olanzapine was obtained as a gift from Incepta Pharmaceuticals Ltd., Bangladesh. Carrageenan powder was purchased from Sigma Aldrich, Germany. Diclofenac sodium, 0.9% saline and disposable syringes were obtained from the Department of Pharmaceutical Sciences, North South University, Bangladesh.

Drying and pulverization

The fresh leaves of the plant Aphanamixis polystachya were washed with water to remove adhering dirt. Then the leaves were cut into small pieces and separated. Finally, the leaves were dried for seven days. After complete drying, the leaves were pulverized into…

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