Animals
Male homozygous (Tg4–42 + /+), hemizygous (Tg4–42 + /−) and non-transgenic wild type (WT) control mice were bred and housed in individually ventilated cages in a controlled environment on a 12/12-h light/dark cycle (lights on from 6 a.m.–6 p.m.), with humidity between 40 to 70%, temperature of approximately 21 °C and food and water ad libitum in an AAALAC accredited animal facility. All animal studies adhered to the Austrian law for the care and use of laboratory animals.
Mice were deeply anesthetized by i.p. injection of 600 mg/kg pentobarbital at the age of 6 months and transcardially perfused with physiological (0.9%) saline. Thereafter, brains were removed, hemisected and subdivided into different brain areas, immediately frozen on dry ice and stored at −80 °C.
Targeted locus amplification and sequence alignment
Targeted Locus Amplification (TLA) of…
