Mice
Human APOE targeted replacement mice were first developed by Sullivan et al.28,29 and were acquired from Dr. Sullivan, or from Taconic Biosciences. All mice used in this study were treated in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and approved by the Columbia University Medical Center and the Nathan Kline Institute’s Institutional Animal Care and Use Committee (IACUC).
RNA-sequencing
Transcriptomics analysis of aged APOE mice was performed as previously described30. Briefly, male mice expressing human APOE3/3 (10 mice) or APOE3/4 (19 mice) were aged to 14–15 months, at which point they were sacrificed by cervical dislocation, and brain tissues containing the EC and PVC were dissected and snap frozen on dry-ice. Brain tissues were stored in RNase-free eppendorf tubes at −80 °C prior to extraction. Total RNA was…
