Protein preparation
The Aβ was purchased from American peptide (Aβ 1–42, 1.0 mg). 1 mg of amyloid pure peptide was dissolved in 100% 1, 1, 1, 3, 3, 3-Hexafluoro-2-propanol (HFIP) to a concentration of 2 mg/ml and incubated at room temperature until a clear solution was formed. The solution was then dried under a nitrogen stream before dissolved once more in HFIP and sonicated. 100 μl of solution was then aliquoted into an eppendorf and stored at −80 °C. When required the solution was thawed and left open in a fume hood overnight to evaporate the HFIP, leaving a peptide pellet. The peptide was dissolved in 10 μl of 100%DMSO and then transferred to a new eppendorf with DMEM.
Protein aggregation
Aβ oligomers were prepared by first diluting in DMEM buffer (DMEM + 1% FCS + 2 mM l-glutamine). The aggregation mixture was incubated for 6 h at 37 °C with…
