Reagents
PU-AD, 131I-PU-AD, 124I-PU-AD, PU-beads (PU-bait) and control beads (CB), and PU-FITC were synthesized using previously reported methods22,58.
Mouse cohorts
Two separate cohorts of transgenic mice were used in this study: (1) male and female hemizygous PS19 harbouring P301S in which the mouse prion promoter drives overexpression of the human T34 isoform of tau (1N4R) which carries the P301S mutation26 and (2) male and female 3xTg-AD59. 3xTg-AD mice are widely used as a model of AD; they express human APPswe, PS1M146V, and tauP301L and progressively develop both Aβ and tau pathology in disease-relevant brain regions and in an age-dependent manner59. The PS19 strain breeds as hemizygotes and the non-transgene containing offspring were used as control animals for transgenic littermates. The 3xTg-AD model was generated using mice of a mixed background; C57BL/6 and 129S1/Sv. Some…
