Animals
In this study, 215 6hTau mice (C57B6 genetic background, from four generations), 75 T44mTauKO mice (C57BL/6 genetic background, from three generations), 85 WT mice (C57BL/6 genetic background), 6 T44 mice (B6D2F1 genetic background, from one generation), and 6 hT-PAC mice (C57B6 genetic background, from one generation) were used. 6hTau mice were generated by crossing a Tg mouse line expressing the normal Htau gene MAPT on a Mtau gene Mapt KO background (hT-PAC-N+/+;Mapt−/−)7 with a Tg mouse line expressing the human tau gene MAPT bearing a mutation at exon 10 + 14 on Mtau gene Mapt KO background (hT-PAC-E10+14+/−;Mapt−/−). This mouse line was maintained as hT-PAC-N+/+;E10+14+/−;Mapt−/−. 6hTau genotyping was performed by PCR using primers: Htau-E10-S1: 5ʹ-ccaagtgtggctcaaaggat-3ʹ, Htau-E10-A1: 5ʹ-cgcactcacaccacttccta-3ʹ. The PCR products were then…
