Diet, APPswe, and sex shapes the microbiota in a model of AD
We administered a 30% CR diet (with reduction in carbohydrates only) to male and female Tg2576 and nontransgenic WT littermates, initiating the diet at 2.5–3 MO and randomly assigning the diet without prior knowledge of microbiota composition. We sequenced the microbial 16S rRNA gene from longitudinally collected fecal samples at 11 different time points until mice reached 15 MO (n = 15–17 per group, with n = 7–9 per group followed until 15 MO). Differences in overall microbiota communities (β-diversity) were determined by comparing unweighted UniFrac distances. By Permanova testing, we found that microbiota communities differed significantly (p < 0.05) in mice by genotype, diet, and biologic sex (Fig. 1a–e and Supplementary Fig. 1). In addition to three-dimensional visualization of community…
