Optogenetic gamma stimulation rescues memory impairments in an Alzheimer’s disease mouse model

Animals

All procedures were approved by the McGill University Animal Care Committee and the Canadian Council on Animal Care. Male mice expressing a mutant form of the human amyloid protein precursor bearing both the Swedish (K670N/M671L) and the Indiana (V717F) mutations (APPSwInd) under the control of the PDGFB promoter [B6. Cg-Tg(PDGFB-APPSwInd)²⁸, (Jackson laboratory)] were bred with female parvalbumin-cre mice (Jackson Laboratory). This mouse line is termed PVJ20 throughout the current study. A total of n = 87 PVJ20 mice were used in this study (n = 44 PVJ20 + , n = 43 PVJ20−). n = 31 mice were implanted with fiber optic implants to perform combined optogenetics and freely moving behavior, n = 6 mice were implanted with linear silicon probes, n = 40 mice were implanted with electrode arrays, and n = 10 mice were used for patch-clamp…