Intracellular application of α-synuclein oligomers reduces spike frequency by enhancing AHP during multiple spikes in neocortical neurons
To clarify the pathophysiological changes in neuronal activity induced by α-synuclein oligomers, whole-cell recordings were obtained from pyramidal neurons located in slices of the mouse frontal cortex, with the solutions with or without α-synuclein oligomers being infused intracellularly from a patch pipette. We prepared several kinds of solutions with α-synuclein, as described in previous reports18,19, and analyzed the molecular state of α-synuclein solutions by immunoblotting (IB) using anti-α-synuclein antibodies (Fig. 1a). Regarding the solutions with wild-type recombinant α-synuclein, higher-order oligomers were detected only in the solution containing α-synuclein incubated with dopamine for 3 days (Wild type, DA); they were not…
