Animals
All mouse work was approved by the Committee for Animal Care of the Division of Comparative Medicine at the Massachusetts Institute of Technology. 5XFAD (Tg 6799) breeding pairs were acquired from the Mutant Mouse Resource and Research Center (MMRRC) (Stock No. 034848-JAX) and maintained as hemizygous on the BL6 background. Animals were group housed on a 12 h light/dark cycle with Nestlet enrichment and killed at ages 18 M, 12 M, 6 M, 4 M, 2 M, 1 M as noted in the text.
Mouse tissue fixation
Mice were deeply anesthetized with isoflurane (Isoflurane, USP, Piramal Healthcare, Andhra Pradesh, India) and underwent transcardial perfusion with ice-cold 1× PBS (10× stock, Gibco, #70011–044) followed by ice-cold fixative made of 4% paraformaldehyde (32% stock, Electron Microscopy Sciences (EMS), Hatfield, PA, #15714) and 1% glutaraldehyde (10% stock, EMS #16110) in 1×…
