Study design and participants
Participants were from the Neurology Unit (University and Polytechnic Hospital La Fe, Valencia, Spain). Their ages were between 50 and 75 years, and they were classified into early AD (case group) (n = 70) and healthy (control group) (n = 29) according to neuropsychological tests, neuroimaging (nuclear magnetic resonance, computerized axial tomography), and CSF biomarkers (β-amyloid, total tau (t-Tau), phosphorylated tau (p-Tau)).
The study protocol was approved by the Ethics Committee (CEIC) from Health Research Institute La Fe (Valencia, Spain), the methods were carried out in accordance with the relevant guidelines and regulations, and informed consent from all participants was obtained.
Lipid peroxidation compounds
The isoprostanes’ standards 5-F2t-IsoP, 2,3-dinor-15-epi-15-F2t-IsoP, 15(R)-15-F2t-IsoP, 15-F2t-IsoP, 15-E2t-IsoP,…
