Cells and culture
HT22 is a mouse hippocampal neuronal cell line28, and cells were cultured in Dulbecco’s modified Eagle medium (DMEM) supplemented with 15% heat-inactivated fetal bovine serum (FBS) and antibiotics at 37 °C under 5% CO2. HeLa cells were cultured in DMEM supplemented with 10% heat-inactivated FBS, 4 mM L-glutamine and antibiotics at 37 °C under 5% CO2.
Preparation of primary rat neuron-enriched cells
Sprague-Dawley rats (Japan SLC, Inc., Shizuoka, Japan) were maintained in the animal care facility of the Niigata University Brain Research Institute. The experiment using rats was approved by the Animal Use and Care Committee of Niigata University and was carried out in accordance with the institutional guidelines and the guidelines of the National Institutes of Health Guide for the Care and Use of Laboratory Animals (NIH Publications No. 80–23).
Rat primary…
