Animal husbandry and ethics statement
The AB wild-type zebrafish (Danio rerio) was maintained in a fish breeding circulatory system (Haisheng, Shanghai, China) at 28 ± 0.5 °C with a 14 h light: 10 h dark cycle. The embryos were produced by adult spawning. For spawning, two pairs of male and female were placed in tank with shallow water. The male and female were separated by a removable partition and kept overnight. The spawning was triggered by removing the partition with first light in the morning and embryos were collected 2 h later, washed with 0.5 ppm methylene blue and placed in petri dish with Holtfreter’s buffer. The healthy embryos at the same developmental stage were selected and developed to 5-day-old larvae for further measurement. All the experiments were performed with larvae in Holtfreter’s buffer55. Tricaine (0.4% in Holtfreter’s buffer) was used…
