Human brain samples
Human postmortem tissues from control and AD patients were obtained from the Netherlands Brain Bank (Netherlands Institute for Neuroscience, Amsterdam, Netherlands). Written informed consent for brain autopsy specimens for research purposes after death was obtained by the Netherlands Brain Bank. Immunofluorescence was performed using antibodies against Iba-1, NeuN, GFAP, and PLIN2 on 8-μm sections as described below.
Animals
ApoE-targeted replacement mice in which the murine ApoE gene locus was replaced by the human ApoE3 or ApoE4 gene were a gift from Yu Qiu (Shanghai Jiao Tong University School of Medicine, Shanghai, China). TRPV1−/− mice with a C57BL/6J background were a gift from Wei Huang (Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China). ApoE mice were bred with TRPV1−/− mice to generate…
