Formal approval to conduct the current experiments was obtained from the the Dalhousie University Committee on Laboratory Animals (Protocol No. 15-070) and the Dalhousie University Radiation Safety Committee, overseen by the Canadian Nuclear Safety Commission (License No. 07154-2-17.10). [99mTc]HMPAO was synthesized at the Department of Diagnostic Imaging, Nova Scotia Heath using the precursor kit obtained as an unconditional gift from GE Healthcare Canada Inc (Mississauga, ON, Canada).
Animals
Pairs of female WT (C57BL/6 J, Stock Number: 034848-JAX-WT-F) and male transgenic hemizygous 5XFAD mice (B6.Cg-Tg(APPSwFlLon,PSEN1*M146L*L286V)6799Vas/Mmjax, Stock Number: 034848-JAX-HET-M) were obtained from the Mutant Mouse Resource & Research Centers and cared for according to the guidelines set forth by the Canadian Council on Animal Care and as described previously14. The WT strain (but…
