Reagents and antibodies
Anti-MITOL rabbit polyclonal antibody was described previously14. Anti-APP C-terminal, anti-α-tubulin, and anti-β-actin antibodies were from Sigma. Anti-Aβ (D54D2), anti-synaptophysin, and anti-presenilin 1 (PS1) antibodies were from Cell Signaling. Anti-normal mouse IgG and anti-Tom20 antibodies were from Santa Cruz Biotechnology. The anti-PSD-95 antibody was from Abcam. Anti-Aβ (6E10) was from Covance. Anti-human Aβ E22P (11A1) antibody was from IBL. A11, OC antibodies were from Thermo Fisher Scientific. Anti-Iba1 was from Wako. Bis-ANS was from Cayman Chemical Company. DAPT and Aβ40 were from PEPTIDE INSTITUTE, INC. Aβ40 was dissolved in 0.02% ammonia solution at 300 µM. To obtain seed-free Aβ40 monomers, these Aβ solutions were centrifuged at 200,000 × g for 3 h at 4 °C and the supernatants were collected. Thus, 0.02% ammonia solution was…
