Posterior basolateral amygdala to ventral hippocampal CA1 drives approach behaviour to exert an anxiolytic effect

a/pBLA innervates vCA1 along its superficial to deep axis

To map the innervation between aBLA or pBLA and vCA1, we first used trans-synaptic virus-delivered anterograde trackers21 (GFP-H129G4 and mCherry-H129-R4) to outline the circuits in physiological conditions. After injecting GFP-H129-G4 into aBLA and pBLA, respectively (Supplementary Fig. 1a, f, i), we observed robust GFP expression in vCA1 but not in dorsal and intermediate hippocampal CA1 (Supplementary Fig. 1a–g). Interestingly, the innervation pattern was very different between aBLA and pBLA, i.e., aBLA predominantly innervated the deep layer (close to stratum oriens) (Supplementary Fig. 1d, k), while the pBLA innervated the superficial layer (close to stratum radiatum) (Supplementary Fig. 1h, l) of vCA1 pyramidal cells (PCs). This innervation pattern was substantially recapitulated when GFP-H129G4 (green) and…

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