Development of probes to detect a second N-terminal cleavage site in NOTCH3
We hypothesized that in addition to the first Asp-Pro sequence of NOTCH3, additional Asp-Pro sequences of the protein could be vulnerable to proteolysis. The current work focuses on the Asp-Pro sequence that separates EGF-like domains 2 and 3 in NOTCH3 (Fig. 1). Cleavage at this novel site is predicted to generate a novel C-terminal epitope that terminates in an aspartic acid residue (human residue Asp121; Fig. 1). In order to detect cleavage at this novel site, monoclonal antibodies specific for the neo-epitope created by this specific cleavage were generated.
Domain structure and primary amino acid sequence of the N-terminus of NOTCH3. (A) Schematic representation of NOTCH3 protein showing N-terminal series of 34 EGF-like domains that compose the bulk of the ectodomain. The vast majority of mutations…
