Skin cells from frontotemporal dementia patients may reveal disease mechanisms and biomarkers for drug research

Skin cells from frontotemporal dementia patients may reveal disease mechanisms and biomarkers for drug research
Fibroblasts of C9-HRE carriers show unaltered C9orf72 levels, express RNA foci, but do not display DPR proteins. a TaqMan assay from fibroblast RNA for C9orf72 all variants. b TaqMan assay from fibroblast RNA for isoform A specific transcript levels. For (a) and (b), data are shown as mean of data points in one biological replicate ± SEM. One-way ANOVA followed by Sidak’s multiple comparison test was performed. Only p values that were significant in the post hoc test are indicated in the graph. c A representative Western blot of the total protein lysates of fibroblasts from a control subject (Con), FTLD patient without the C9-HRE (C9−), and FTLD patient with the C9-HRE (C9+). Cells were treated with 10 μM lactacystin (Lact.) overnight to block protein degradation through UPS. Poly-ubiquitinated proteins…

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