Cellular culture and transfection
Murine Neuroblastoma N2a cells were stably transfected with pcDNA 3.1 Zeo + vector with resistance to Zeocin. 1 µg of 4 different plasmids: Mock (empty vector), human PS1WT, PS1E280A and PS1Δ9 were used to transfect the cells with lipofectamineTM 2000 (Thermo Fisher Scientific, Schwerte, Germany), according to manufacturer’s instructions. The clones were established by selection with Zeocin (Invitrogen, Carlsbad, CA, USA). The dose used for selection was 200 µg/ml. Positive clones were isolated after approximately 30 days. Overexpression of human PS1 was assessed via western blot and qPCR for human PS1 (Fig. S1A–C). Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and treated with different reagents to induce cellular stress and death (see below). PS KO−/− MEFs and PS…
