Peripheral inflammation aggravated the neurological dysfunction in SAD in a NLRP3-dependent manner
In open field test, time spent in the region of interest (ROI) was significantly decreased in STZ group in Cre− mice compared with that in sham group, it was further decreased in STZ + lipopolysaccharide (LPS) group, whereas increased in STZ + MCC950 group (Fig. 1B). Time spent in the ROI in STZ and STZ + LPS groups was significantly increased in Cre+ mice compared with that in Cre− mice. These results indicated that STZ injection caused anxiety, which was aggravated by peripheral inflammation but attenuated by inhibition or depletion of microglial NLRP3.
A Time point of the schematic design. B Analysis of time spending in the central area (Region of interest, ROI) in the open field test. C…
