Amyloid pathology–produced unexpected modifications of calcium homeostasis in hippocampal subicular dendrites

Fig. 4

Estimation of the Ca²⁺-buffering capacity in apical dendrites of subicular BC neurons from different genotypes in old mice. (A) Representative Ca²⁺ transients evoked with a single depolarization using different concentrations of OG1 in the pipette (12.5, 25, 50, and 100 μM, as indicated). Mono-exponential fitting is in dark blue. Note that, as expected, the decay time constant is slower with higher OG1 concentrations in all neurons recorded. (B) Mean + SEM of peak Ca²⁺ concentrations at different concentrations of OG1 in the three genotypes (indicated in the graph), elicited with a single depolarization (1AP) (3 ms, from −70 mV to +10 mV). (C) Mean + SEM of Ca²⁺ transients decay time constants elicited with 3 rapid depolarizations (3AP) (100 Hz, 3 ms, from −70 mV to +10 mV). Data obtained from these transients were used to estimate the Ca²⁺-buffering capacity…